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排序方式: 共有610条查询结果,搜索用时 271 毫秒
41.
Richard Ward Christos Pliotas Emma Branigan Christian Hacker Akiko Rasmussen Gregor Hagelueken Ian R. Booth Samantha Miller John Lucocq James H. Naismith Olav Schiemann 《Biophysical journal》2014
Mechanosensitive channel proteins are important safety valves against osmotic shock in bacteria, and are involved in sensing touch and sound waves in higher organisms. The mechanosensitive channel of small conductance (MscS) has been extensively studied. Pulsed electron-electron double resonance (PELDOR or DEER) of detergent-solubilized protein confirms that as seen in the crystal structure, the outer ring of transmembrane helices do not pack against the pore-forming helices, creating an apparent void. The relevance of this void to the functional form of MscS in the bilayer is the subject of debate. Here, we report PELDOR measurements of MscS reconstituted into two lipid bilayer systems: nanodiscs and bicelles. The distance measurements from multiple mutants derived from the PELDOR data are consistent with the detergent-solution arrangement of the protein. We conclude, therefore, that the relative positioning of the transmembrane helices is preserved in mimics of the cell bilayer, and that the apparent voids are not an artifact of detergent solution but a property of the protein that will have to be accounted for in any molecular mechanism of gating. 相似文献
42.
Anne Sverdrup-Thygeson Olav Skarpaas Frode Ødegaard 《Biodiversity and Conservation》2010,19(3):837-852
In this study we investigated hollow oaks (Quercus robur, Q. petrea) situated in open landscapes and in forests in Norway in northern Europe, and compared their importance for rare and threatened beetles (Coleoptera). Old, hollow oak trees, both in parks and in forests, were extremely rich in red-listed beetles, and hosted a high proportion of threatened species. The proportion of oak associated species and the mean number of red-listed beetle species per tree was similar in the two site types, but rarefaction showed that for a certain number of individuals, oaks in forests had more threatened and near-threatened species than oaks in parks. The species composition also differed between site types: Park oaks had a higher proportion of species associated with hollows and animal nests, whereas in forests, there was a higher proportion of species depending on dead oak wood in general. Four factors were significant in explaining the richness of red-listed beetles in our study: Tree circumference, cavity decay stage, proportion of oak in the surroundings, and coarse woody debris (CWD) in the surroundings. Forest oaks were smaller, but they still trapped a species richness comparable to that of the larger park oaks—probably a result of high amounts of CWD in the surroundings. We show that oaks in open landscapes and oaks in forest have only partly overlapping beetle assemblages and, thus, cannot be substituted in conservation. Planning for conservation of red-listed beetles associated with this key habitat demands a large scale perspective, both in space and time, as the surroundings have important effects on associated threatened and near threatened species. 相似文献
43.
Moose summer and winter diets along a large scale gradient of forage availability in southern Norway
Studies on dietary functional responses in large herbivores are traditionally conducted by following individual animals. The
method is very time-consuming, and hence, typically provides only a narrow array of forage species compositions. Here we use
a range level approach to look at moose (Alces alces) selectivity for and utilization of forage species in relation to availability in both summer and winter. We compare 12 Norwegian
ranges representing a large scale gradient in plant communities. The most important forage species in the diet were birches
(Betula spp., comprising 43% of all trees browsed in summer and 27% in winter), rowan (Sorbus aucuparia, 25% of trees browsed in summer, 37% in winter), and bilberry (Vaccinum myrtillus, 42% of herbaceous epidermal fragments in summer feces). Selectivity for birches was positively related to its availability
and negatively related to availability of rowan, Salix spp., and aspen (Populus tremula) together (all more selected for than birches). Multiple regression models including availability of several forage species
were thus superior to single-species models in explaining the diet content of main forage plants. Selectivity for birches
was also stronger in summer than in winter, while the opposite pattern was found for rowan. The finding is relevant for our
evaluation of the quality of summer and winter ranges, and hence, their relative influence on population productivity. Our
study underlines the need to incorporate species composition of available forage when quantifying dietary functional responses
in selective herbivores such as moose. Furthermore, care should be taken when extrapolating data on moose diet across ranges
or seasons. 相似文献
44.
Henrik Sundh Bjørn Olav Kvamme Frode Fridell Rolf Erik Olsen Tim Ellis Geir Lasse Taranger Kristina Sundell 《BMC physiology》2010,10(1):22
Background
Fish farmed under high intensity aquaculture conditions are subjected to unnatural environments that may cause stress. Therefore awareness of how to maintain good health and welfare of farmed fish is important. For Atlantic salmon held in sea cages, water flow, dissolved oxygen (DO) levels and temperature will fluctuate over time and the fish can at times be exposed to detrimentally low DO levels and high temperatures. This experimental study investigates primary and secondary stress responses of Atlantic salmon post smolts to long-term exposure to reduced and fluctuating DO levels and high water temperatures, mimicking situations in the sea cages. Plasma cortisol levels and cortisol release to the water were assessed as indicators of the primary stress response and intestinal barrier integrity and physiological functions as indicators of secondary responses to changes in environmental conditions. 相似文献45.
Plantaricin A (PlnA) is a 26-mer peptide pheromone with membrane-permeabilizing, strain-specific antibacterial activity, produced
by Lactobacillus plantarum C11. We investigated the membrane-permeabilizing effects of PlnA on cultured cancerous and normal rat anterior pituitary
cells using patch-clamp techniques and microfluorometry (fura-2). Cancerous cells displayed massive permeabilization within
5 s after exposure to 10–100 μm PlnA. The membrane depolarized to nearly 0 mV, and the membrane resistance decreased to a mere fraction of the initial value after less than 1 min. In outside-out membrane
patches, 10 μm PlnA induced membrane currents reversing at 0 mV, which is compatible with an unspecific conductance increase. The d and l forms of the peptide had similar potency, indicating a nonchiral mechanism for the membrane-permeabilizing effect. Surprisingly,
inside-out patches were insensitive to 1 mm PlnA. Primary cultures of normal rat anterior pituitary cells were also insensitive to the peptide. Thus, PlnA differentiates
between plasma membranes and membrane leaflets. Microfluorometric recordings of [Ca2+]
i
and cytosolic concentration of fluorochrome verified the rapid permeabilizing effect of PlnA on cancerous cells and the insensitivity
of normal pituitary cells. 相似文献
46.
Wilson A Ardiet DL Saner C Vilain N Beermann F Aguet M Macdonald HR Zilian O 《Journal of immunology (Baltimore, Md. : 1950)》2007,178(11):6746-6751
The mammalian ortholog of the conserved Drosophila adaptor protein Numb (Nb) and its homolog Numblike (Nbl) modulate neuronal cell fate determination at least in part by antagonizing Notch signaling. Because the Notch pathway has been implicated in regulating hemopoietic stem cell self-renewal and T cell fate specification in mammals, we investigated the role of Nb and Nbl in hemopoiesis using conditional gene targeting. Surprisingly simultaneous deletion of both Nb and Nbl in murine bone marrow precursors did not affect the ability of stem cells to self-renew or to give rise to differentiated myeloid or lymphoid progeny, even under competitive conditions in mixed chimeras. Furthermore, T cell fate specification and intrathymic T cell development were unaffected in the combined absence of Nb and Nbl. Collectively our data indicate that the Nb family of adaptor proteins is dispensable for hemopoiesis and lymphopoiesis in mice, despite their proposed role in neuronal stem cell development. 相似文献
47.
Aging without functional senescence in honey bee workers 总被引:2,自引:0,他引:2
48.
After a marked decline at the beginning of the 1900s, the arctic fox Alopex lagopus population in Fennoscandia has remained at a very low level. We suggest that the main cause for the population crash was
winter starvation caused by (1) over-hunting of reindeer Rangifer tarandus populations, and thus reduced carcass availability in the mountains, and (2) increased interspecific competition for these
carcasses because of increased invasion of red foxes Vulpes vulpes from lower altitudes. The failure of arctic fox populations to recover, despite increasing reindeer populations in the mid
1900s, can be explained by a concurrent strong increase in red fox numbers. Analyses of countywide hunting statistics from
Norway 1891–1920 suggest that there actually was an increase in red fox numbers in the period of arctic fox decline, and that
the increase in reindeer populations from the 1920s to the 1950s was accompanied by a new increase in red fox numbers. We
conclude that restoring arctic fox populations most likely will require a substantial and lasting reduction of red fox populations. 相似文献
49.
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